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Chick Embryo: A Preclinical Model for understanding ischemia reperfusion model

Amit Kumar Shrivastava1, Syed Shadab Raza2, Eram Fauzia3, Manish Kumar4, Parth Garg

Ischemia-reperfusion (I/R)-related disorders, such as stroke, myocardial infarction, and other peripheral vascular disease, are among the most frequent causes of disease and death. Tissue injury or death may result from the initial ischemic insult, primarily determined by the magnitude and duration of the interruption in blood supply then by the next reperfusion-it induced damage. Various in vitro and in vivo models are currently available to review I/R mechanism within the brain and other tissues. Here, we developed an in ovo Hook model of I/R by occluding and releasing the proper vitelline artery of a chick embryo at 72 h of development. To validate the model and elucidate various underlying survival and death mechanisms, we employed imaging (Doppler blood flow imaging), biochemical, and blotting techniques and evaluated the necrobiosis mechanism: autophagy and inflammation caused by I/R. In conclusion, this model is beneficial in parallel with established in vitro and in vivo I/R models to know the mechanisms of I/R development and its treatment.